serotype b r540 (ATCC)
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serotype b r540
Serotype B R540, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serotype+b+r540/Haemophilus+influenzae+(Lehmann+and+Neumann)+Winslow+et+al/pmc00107485-112-186-189
Average 94 stars, based on 50 article reviews
Serotype B R540, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/serotype+b+r540/Haemophilus+influenzae+(Lehmann+and+Neumann)+Winslow+et+al/pmc00107485-112-186-189
Average 94 stars, based on 50 article reviews
serotype b r540 - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae"
Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae
Journal:
doi:
Figure Legend Snippet: Bacterial strains and plasmids
Techniques Used: Plasmid Preparation, Clone Assay, Amplification, Subcloning, Infection
Related Articles
Purification:Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae Article Snippet: .. Growth of E. coli and H. influenzae and purification of E. coli plasmids were as previously described ( 25 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Source a Relevant properties Reference(s) E. coli DH5α GIBCO BRL Host for E. coli plasmids E. coli plasmids Bluescript pSK − Stratagene pHic Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of Alu I partial digestion fragment into Sma I site of pSK− This study pMCC1 Pst I (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA , hifB , and the 5′ end of hifC This study; 13 H. influenzae strains Eagan (E1A) CSF from meningitis patient Type b, streptomycin resistant, virulent in infant rat model 38 Rd (R906) = Goodgal Nonencapsulated derivative of type d strain; avirulent in rat model 8 INT1 Blood from meningitis patient Nonencapsulated; biotype V; virulent in rat model 31 R539 (ATCC 9006) ATCC Reference strain of serotype a R538 (ATCC 9795) ATCC Reference strain of Plasmid Preparation:Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae Article Snippet: .. Growth of E. coli and H. influenzae and purification of E. coli plasmids were as previously described ( 25 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Source a Relevant properties Reference(s) E. coli DH5α GIBCO BRL Host for E. coli plasmids E. coli plasmids Bluescript pSK − Stratagene pHic Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of Alu I partial digestion fragment into Sma I site of pSK− This study pMCC1 Pst I (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA , hifB , and the 5′ end of hifC This study; 13 H. influenzae strains Eagan (E1A) CSF from meningitis patient Type b, streptomycin resistant, virulent in infant rat model 38 Rd (R906) = Goodgal Nonencapsulated derivative of type d strain; avirulent in rat model 8 INT1 Blood from meningitis patient Nonencapsulated; biotype V; virulent in rat model 31 R539 (ATCC 9006) ATCC Reference strain of serotype a R538 (ATCC 9795) ATCC Reference strain of Clone Assay:Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae Article Snippet: .. Growth of E. coli and H. influenzae and purification of E. coli plasmids were as previously described ( 25 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Source a Relevant properties Reference(s) E. coli DH5α GIBCO BRL Host for E. coli plasmids E. coli plasmids Bluescript pSK − Stratagene pHic Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of Alu I partial digestion fragment into Sma I site of pSK− This study pMCC1 Pst I (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA , hifB , and the 5′ end of hifC This study; 13 H. influenzae strains Eagan (E1A) CSF from meningitis patient Type b, streptomycin resistant, virulent in infant rat model 38 Rd (R906) = Goodgal Nonencapsulated derivative of type d strain; avirulent in rat model 8 INT1 Blood from meningitis patient Nonencapsulated; biotype V; virulent in rat model 31 R539 (ATCC 9006) ATCC Reference strain of serotype a R538 (ATCC 9795) ATCC Reference strain of Polymerase Chain Reaction:Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae Article Snippet: .. Growth of E. coli and H. influenzae and purification of E. coli plasmids were as previously described ( 25 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Source a Relevant properties Reference(s) E. coli DH5α GIBCO BRL Host for E. coli plasmids E. coli plasmids Bluescript pSK − Stratagene pHic Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of Alu I partial digestion fragment into Sma I site of pSK− This study pMCC1 Pst I (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA , hifB , and the 5′ end of hifC This study; 13 H. influenzae strains Eagan (E1A) CSF from meningitis patient Type b, streptomycin resistant, virulent in infant rat model 38 Rd (R906) = Goodgal Nonencapsulated derivative of type d strain; avirulent in rat model 8 INT1 Blood from meningitis patient Nonencapsulated; biotype V; virulent in rat model 31 R539 (ATCC 9006) ATCC Reference strain of serotype a R538 (ATCC 9795) ATCC Reference strain of Amplification:Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae Article Snippet: .. Growth of E. coli and H. influenzae and purification of E. coli plasmids were as previously described ( 25 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Source a Relevant properties Reference(s) E. coli DH5α GIBCO BRL Host for E. coli plasmids E. coli plasmids Bluescript pSK − Stratagene pHic Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of Alu I partial digestion fragment into Sma I site of pSK− This study pMCC1 Pst I (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA , hifB , and the 5′ end of hifC This study; 13 H. influenzae strains Eagan (E1A) CSF from meningitis patient Type b, streptomycin resistant, virulent in infant rat model 38 Rd (R906) = Goodgal Nonencapsulated derivative of type d strain; avirulent in rat model 8 INT1 Blood from meningitis patient Nonencapsulated; biotype V; virulent in rat model 31 R539 (ATCC 9006) ATCC Reference strain of serotype a R538 (ATCC 9795) ATCC Reference strain of Subcloning:Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae Article Snippet: .. Growth of E. coli and H. influenzae and purification of E. coli plasmids were as previously described ( 25 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Source a Relevant properties Reference(s) E. coli DH5α GIBCO BRL Host for E. coli plasmids E. coli plasmids Bluescript pSK − Stratagene pHic Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of Alu I partial digestion fragment into Sma I site of pSK− This study pMCC1 Pst I (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA , hifB , and the 5′ end of hifC This study; 13 H. influenzae strains Eagan (E1A) CSF from meningitis patient Type b, streptomycin resistant, virulent in infant rat model 38 Rd (R906) = Goodgal Nonencapsulated derivative of type d strain; avirulent in rat model 8 INT1 Blood from meningitis patient Nonencapsulated; biotype V; virulent in rat model 31 R539 (ATCC 9006) ATCC Reference strain of serotype a R538 (ATCC 9795) ATCC Reference strain of Infection:Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae Article Snippet: .. Growth of E. coli and H. influenzae and purification of E. coli plasmids were as previously described ( 25 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Source a Relevant properties Reference(s) E. coli DH5α GIBCO BRL Host for E. coli plasmids E. coli plasmids Bluescript pSK − Stratagene pHic Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of Alu I partial digestion fragment into Sma I site of pSK− This study pMCC1 Pst I (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA , hifB , and the 5′ end of hifC This study; 13 H. influenzae strains Eagan (E1A) CSF from meningitis patient Type b, streptomycin resistant, virulent in infant rat model 38 Rd (R906) = Goodgal Nonencapsulated derivative of type d strain; avirulent in rat model 8 INT1 Blood from meningitis patient Nonencapsulated; biotype V; virulent in rat model 31 R539 (ATCC 9006) ATCC Reference strain of serotype a R538 (ATCC 9795) ATCC Reference strain of |